Androgen-induced kidney hypertrophy in the European bullhead (Cottus sp.): a potential biomarker of androgen exposure.
Identifieur interne : 000580 ( Main/Exploration ); précédent : 000579; suivant : 000581Androgen-induced kidney hypertrophy in the European bullhead (Cottus sp.): a potential biomarker of androgen exposure.
Auteurs : M. Villeret [France] ; C. Blanchard [France] ; L. R. Le Rohic [France] ; Laure Wiest [France] ; Emmanuelle Vulliet [France] ; C. Minier [France] ; Jean-Marc Porcher [France] ; Wilfried Sanchez [France]Source :
Abstract
The present work was designed to identify an androgeno-regulated signal in the kidney of the European bullhead (Cottus sp.) and to characterise response patterns prior to laboratory and field applications to address androgenic potential of chemicals. In a first step, gender dependent ultrastructural modifications of kidney were quantified using the Kidney Epithelium Height (KEH) measurement. During breeding period, male bullheads exhibited an increased KEH value of 20.96 ± 3.57 μm compared to female (16.96 ± 1.28 μm). No gender difference was recorded in non-breeding bullheads. In a second step, bullheads out of breeding period were exposed 14 or 21 days to different concentrations of androgens (0.5 or 5 μg/L of trenbolone (Tb), 1 or 10 μg/L of 11-ketotestosterone (11KT), 0.5 or 5 μg/L of Spironolactone (SPI), 0.5, 5 or 50 μg/L of Dihydrotestosterone (DHT)), estrogen (0.05 or 0.5 μg/L of Ethinyl-estradiol (EE2)) or heavy metal (1 or 10 μg/L of Cadmium (Cd)). Results showed that androgens are able to induce kidney hypertrophy. Trenbolone induced a dose-dependent hypertrophy (KEH= 16.59 ± 0.81 μm at 0.5 μg/L and 21.01 ± 1.76 μm at 5 μg/L vs 15.58 ± 0.85 μm for the controls) as the higher concentration of 11-KT (KEH = 17.55 ± 1.48 μm at 10 μg/L) and DHT (17.43 ± 1.77 μm at 50 μg/L). SPI and Cd have no effect on kidney ultrastructure. In bullheads exposed to EE2, a trend to decrease of KEH was observed. In a next step, wild bullheads were electrofished in 9 streams located in the North of France and characterised by various contamination levels. KEH responses don't allow to discriminate both reference (KEH = 16.03.± 1.25 μm) and contaminated (KEH = 16.17 ± 1.08 μm) sites. This work highlights an androgen-regulated signal in the kidney of the European bullhead. However, kidney hypertrophy appears as few sensitive and time consuming indicators. Also, identification of associated protein and/or RNA modifications could provide a relevant biomarker of androgen exposure.
Url:
Affiliations:
- France
- Auvergne-Rhône-Alpes, Haute-Normandie, Rhône-Alpes, Région Normandie
- Le Havre, Lyon
- Université Claude Bernard Lyon 1, Université de Lyon, Université du Havre
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: 000042
- to stream Hal, to step Curation: 000042
- to stream Hal, to step Checkpoint: 000307
- to stream Main, to step Merge: 000584
- to stream Main, to step Curation: 000580
Le document en format XML
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Lyon</orgName><orgName type="acronym">ENS Lyon</orgName><desc><address><addrLine>15 parvis René Descartes - BP 7000 - 69342 Lyon Cedex 07</addrLine><country key="FR"></country></address><ref type="url">http://www.ens-lyon.eu/</ref></desc></org></tutelle><tutelle active="#struct-194495" type="indirect"><org type="institution" xml:id="struct-194495" status="VALID"><orgName>Université Claude Bernard Lyon 1</orgName><orgName type="acronym">UCBL</orgName><desc><address><addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lyon1.fr/</ref></desc></org></tutelle><tutelle active="#struct-301088" type="indirect"><org type="institution" xml:id="struct-301088" status="VALID"><orgName>PRES Université de Lyon</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="UMR5280" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Lyon</settlement><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region></placeName><orgName type="university">Université Claude Bernard Lyon 1</orgName><orgName type="institution" wicri:auto="newGroup">Université de Lyon</orgName></affiliation></author><author><name sortKey="Minier, C" sort="Minier, C" uniqKey="Minier C" first="C." last="Minier">C. Minier</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-131790" status="VALID"><orgName>Laboratoire d'Ecotoxicologie et des Milieux Aquatiques</orgName><orgName type="acronym">LEMA</orgName><desc><address><addrLine>UFR de Sciences et Techniques, Le Havre</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lehavre.fr/ulh_services/Laboratoire-d-Ecotoxicologie.html</ref></desc><listRelation><relation name="EA3222" active="#struct-300317" type="direct"></relation></listRelation><tutelles><tutelle name="EA3222" active="#struct-300317" type="direct"><org type="institution" xml:id="struct-300317" status="VALID"><orgName>Université du Havre</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Le Havre</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université du Havre</orgName></affiliation></author><author><name sortKey="Porcher, Jean Marc" sort="Porcher, Jean Marc" uniqKey="Porcher J" first="Jean-Marc" last="Porcher">Jean-Marc Porcher</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-3186" status="VALID"><orgName>Institut National de l'Environnement Industriel et des Risques</orgName><orgName type="acronym">INERIS</orgName><desc><address><country key="FR"></country></address><ref type="url">http://www.ineris.fr/</ref></desc><listRelation><relation active="#struct-300023" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300023" type="direct"><org type="institution" xml:id="struct-300023" status="VALID"><orgName>INERIS</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Sanchez, Wilfried" sort="Sanchez, Wilfried" uniqKey="Sanchez W" first="Wilfried" last="Sanchez">Wilfried Sanchez</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-44968" status="INCOMING"><orgName>Unité d'évaluation des risques écotoxicologiques</orgName><orgName type="acronym">DRC-ECOT</orgName><desc><address><addrLine>parc technologique ALATA BP 2 60550 verneuil en halatte</addrLine><country key="FR"></country></address><ref type="url">http://www.ineris.fr</ref></desc><listRelation><relation active="#struct-300023" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300023" type="direct"><org type="institution" xml:id="struct-300023" status="VALID"><orgName>INERIS</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author></analytic></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present work was designed to identify an androgeno-regulated signal in the kidney of the European bullhead (Cottus sp.) and to characterise response patterns prior to laboratory and field applications to address androgenic potential of chemicals. In a first step, gender dependent ultrastructural modifications of kidney were quantified using the Kidney Epithelium Height (KEH) measurement. During breeding period, male bullheads exhibited an increased KEH value of 20.96 ± 3.57 μm compared to female (16.96 ± 1.28 μm). No gender difference was recorded in non-breeding bullheads. In a second step, bullheads out of breeding period were exposed 14 or 21 days to different concentrations of androgens (0.5 or 5 μg/L of trenbolone (Tb), 1 or 10 μg/L of 11-ketotestosterone (11KT), 0.5 or 5 μg/L of Spironolactone (SPI), 0.5, 5 or 50 μg/L of Dihydrotestosterone (DHT)), estrogen (0.05 or 0.5 μg/L of Ethinyl-estradiol (EE2)) or heavy metal (1 or 10 μg/L of Cadmium (Cd)). Results showed that androgens are able to induce kidney hypertrophy. Trenbolone induced a dose-dependent hypertrophy (KEH= 16.59 ± 0.81 μm at 0.5 μg/L and 21.01 ± 1.76 μm at 5 μg/L vs 15.58 ± 0.85 μm for the controls) as the higher concentration of 11-KT (KEH = 17.55 ± 1.48 μm at 10 μg/L) and DHT (17.43 ± 1.77 μm at 50 μg/L). SPI and Cd have no effect on kidney ultrastructure. In bullheads exposed to EE2, a trend to decrease of KEH was observed. In a next step, wild bullheads were electrofished in 9 streams located in the North of France and characterised by various contamination levels. KEH responses don't allow to discriminate both reference (KEH = 16.03.± 1.25 μm) and contaminated (KEH = 16.17 ± 1.08 μm) sites. This work highlights an androgen-regulated signal in the kidney of the European bullhead. However, kidney hypertrophy appears as few sensitive and time consuming indicators. Also, identification of associated protein and/or RNA modifications could provide a relevant biomarker of androgen exposure.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Haute-Normandie</li><li>Rhône-Alpes</li><li>Région Normandie</li></region><settlement><li>Le Havre</li><li>Lyon</li></settlement><orgName><li>Université Claude Bernard Lyon 1</li><li>Université de Lyon</li><li>Université du Havre</li></orgName></list><tree><country name="France"><noRegion><name sortKey="Villeret, M" sort="Villeret, M" uniqKey="Villeret M" first="M." last="Villeret">M. Villeret</name></noRegion><name sortKey="Blanchard, C" sort="Blanchard, C" uniqKey="Blanchard C" first="C." last="Blanchard">C. Blanchard</name><name sortKey="Le Rohic, L R" sort="Le Rohic, L R" uniqKey="Le Rohic L" first="L. R." last="Le Rohic">L. R. Le Rohic</name><name sortKey="Minier, C" sort="Minier, C" uniqKey="Minier C" first="C." last="Minier">C. Minier</name><name sortKey="Porcher, Jean Marc" sort="Porcher, Jean Marc" uniqKey="Porcher J" first="Jean-Marc" last="Porcher">Jean-Marc Porcher</name><name sortKey="Sanchez, Wilfried" sort="Sanchez, Wilfried" uniqKey="Sanchez W" first="Wilfried" last="Sanchez">Wilfried Sanchez</name><name sortKey="Vulliet, Emmanuelle" sort="Vulliet, Emmanuelle" uniqKey="Vulliet E" first="Emmanuelle" last="Vulliet">Emmanuelle Vulliet</name><name sortKey="Wiest, Laure" sort="Wiest, Laure" uniqKey="Wiest L" first="Laure" last="Wiest">Laure Wiest</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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